Tian Liu
06/17/2024, 10:19 PMError executing process > 'NFCORE_CHIPSEQ:CHIPSEQ:PHANTOMPEAKQUALTOOLS (A4_H_H3K27Ac)'
Caused by:
Process `NFCORE_CHIPSEQ:CHIPSEQ:PHANTOMPEAKQUALTOOLS (A4_H_H3K27Ac)` terminated with an error exit status (1)
Command executed:
RUN_SPP=`which run_spp.R`
Rscript -e "library(caTools); source(\"$RUN_SPP\")" -c="A4_H_H3K27Ac.mLb.clN.sorted.bam" -savp="A4_H_H3K27Ac.spp.pdf" -savd="A4_H_H3K27Ac.spp.Rdata" -out="A4_H_H3K27Ac.spp.out" -p=6
cat <<-END_VERSIONS > versions.yml
"NFCORE_CHIPSEQ:CHIPSEQ:PHANTOMPEAKQUALTOOLS":
phantompeakqualtools: 1.2.2
END_VERSIONS
Command exit status:
1
Command output:
################
ChIP data: A4_H_H3K27Ac.mLb.clN.sorted.bam
Control data: NA
strandshift(min): -500
strandshift(step): 5
strandshift(max) 1500
user-defined peak shift NA
exclusion(min): 10
exclusion(max): NaN
num parallel nodes: 6
FDR threshold: 0.01
NumPeaks Threshold: NA
Output Directory: .
narrowPeak output file name: NA
regionPeak output file name: NA
Rdata filename: A4_H_H3K27Ac.spp.Rdata
plot pdf filename: A4_H_H3K27Ac.spp.pdf
result filename: A4_H_H3K27Ac.spp.out
Overwrite files?: FALSE
Reading ChIP tagAlign/BAM file A4_H_H3K27Ac.mLb.clN.sorted.bam
opened /tmp/RtmpwcvaIx/A4_H_H3K27Ac.mLb.clN.sorted.tagAlign29344786f7
done. read 55416748 fragments
ChIP data read length 150
Calculating peak characteristics
Minimum cross-correlation value 0.3306441
Minimum cross-correlation shift 1500
Top 3 cross-correlation values NA
Top 3 estimates for fragment length NA
Window half size NA
Phantom peak location 140
Phantom peak Correlation 0.6661341
Normalized Strand cross-correlation coefficient (NSC) NA
Relative Strand cross-correlation Coefficient (RSC) NA
Command error:
WARNING: Skipping mount /cluster/software/modules-sw/singularity/ce-3.9.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container
Loading required package: Rcpp
################
ChIP data: A4_H_H3K27Ac.mLb.clN.sorted.bam
Control data: NA
strandshift(min): -500
strandshift(step): 5
strandshift(max) 1500
user-defined peak shift NA
exclusion(min): 10
exclusion(max): NaN
num parallel nodes: 6
FDR threshold: 0.01
NumPeaks Threshold: NA
Output Directory: .
narrowPeak output file name: NA
regionPeak output file name: NA
Rdata filename: A4_H_H3K27Ac.spp.Rdata
plot pdf filename: A4_H_H3K27Ac.spp.pdf
result filename: A4_H_H3K27Ac.spp.out
Overwrite files?: FALSE
Reading ChIP tagAlign/BAM file A4_H_H3K27Ac.mLb.clN.sorted.bam
opened /tmp/RtmpwcvaIx/A4_H_H3K27Ac.mLb.clN.sorted.tagAlign29344786f7
done. read 55416748 fragments
ChIP data read length 150
Calculating peak characteristics
Minimum cross-correlation value 0.3306441
Minimum cross-correlation shift 1500
Top 3 cross-correlation values NA
Top 3 estimates for fragment length NA
Window half size NA
Phantom peak location 140
Phantom peak Correlation 0.6661341
Normalized Strand cross-correlation coefficient (NSC) NA
Relative Strand cross-correlation Coefficient (RSC) NA
Error in if ((crosscorr$rel.phantom.coeff >= 0) & (crosscorr$rel.phantom.coeff < :
missing value where TRUE/FALSE needed
Calls: source -> withVisible -> eval -> eval
Execution halted
Work dir:
/beevol/home/liutian/Documents/cut_and_run/Foreman/ChIP_Seq/fastq_files/work/fe/3cc1e598f71ada27f13077cd426583
Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
My script:
nextflow run nf-core/chipseq
-profile singularity
--input Foreman_lab_ChIPseq.csv
--genome GRCh38
--narrow_peak false
--read_length 150
--macs_gsize 2862089864
--outdir $OUTPUT
--skip_spp
-resume
What should I do to fix this error? Thanks!Jose Espinosa-Carrasco
06/19/2024, 8:17 AM