```ERROR ~ Error executing process > 'NFCORE_ME...
# metatdenovo
e
Copy code
ERROR ~ Error executing process > 'NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH (rnaspades.prokka)'

Caused by:
  Process `NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH (rnaspades.prokka)` terminated with an error exit status (1)

Command executed:

  rc=0
  mkdir contigs
  gunzip -c prokka.faa.gz > ./contigs/proteins.faa
  EUKulele \
      -m mets \
      --database gtdb \
      --protein_extension .faa \
      --reference_dir eukulele \
      -o rnaspades.prokka \
      --CPUs 12 \
      -s \
      contigs || rc=$?
  
  gzip rnaspades.prokka/mets_full/diamond/*.out
  gzip rnaspades.prokka/taxonomy_counts/*.csv
  gzip rnaspades.prokka/taxonomy_estimation/*.out
  
  cat <<-END_VERSIONS > versions.yml
  "NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH":
      eukulele: $(echo $(EUKulele --version 2>&1) | sed -n 's/.* \([0-9]\+\.[0-9]\+\.[0-9]\+\).*/\1/p')
  END_VERSIONS
  
  if [ $rc -le 1 ]; then
      exit 0
  else
      exit $rc;
  fi

Command exit status:
  1

Command output:
  All reference files for GTDB downloaded to eukulele/gtdb
  Running EUKulele with command line arguments, as no valid configuration file was provided.
  Setting things up...
  Could not successfully install all external dependent software.
  Check DIAMOND, BLAST, BUSCO, and TransDecoder installation.
  ['proteins']
  Specified reference directory, reference FASTA, and protein map/taxonomy table not found. Using database in location: eukulele/gtdb.
  Automatically downloading database gtdb . If you intended to use an existing database folder, be sure a reference FASTA, protein map, and taxonomy table are provided. Check the documentation for details.

Command error:
  mkdir: can't create directory 'eukulele/': No such file or directory
  mkdir: can't create directory 'eukulele/': No such file or directory
  eukulele/gtdb/reference.pep.fa: No such file or directory
  eukulele/gtdb/taxonomy-table.txt: No such file or directory
  All reference files for GTDB downloaded to eukulele/gtdb
  Running EUKulele with command line arguments, as no valid configuration file was provided.
  Setting things up...
  Could not successfully install all external dependent software.
  Check DIAMOND, BLAST, BUSCO, and TransDecoder installation.
  ['proteins']
  Specified reference directory, reference FASTA, and protein map/taxonomy table not found. Using database in location: eukulele/gtdb.
  Automatically downloading database gtdb . If you intended to use an existing database folder, be sure a reference FASTA, protein map, and taxonomy table are provided. Check the documentation for details.
  gzip: rnaspades.prokka/mets_full/diamond/*.out: No such file or directory

Work dir:
  /home/erick/Nextflow/nf-core_metatdenovo/work/b5/d67099bed08afe34efc734777ae227

Tip: you can try to figure out what's wrong by changing to the process work dir and showing the script file named `.command.sh`

 -- Check '.nextflow.log' file for details
d
did you download gtdb before running the pipeline?
e
Hi Danilo! No I didin't... I thought the database was downloaded automatically as in the Ampliseq pipeline. Now I've read the documentation and see that you recommend downloading the database manually and adding the path until the developers fix the bug. I'm going to try this and see if I don't encounter another error.
🤞
Thank you so much!
I will let you know about my progress
👋
d
good luck 🤞
e
I download the database and add the path:
Copy code
--eukulele_dbpath  /home/erick/Descargas/gtdb/
And then I got a new error... A new error is a progress 💪
Copy code
ERROR ~ Error executing process > 'NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH (rnaspades.prokka)'

Caused by:
  Process `NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH (rnaspades.prokka)` terminated with an error exit status (1)

Command executed:

  rc=0
  mkdir contigs
  gunzip -c prokka.faa.gz > ./contigs/proteins.faa
  EUKulele \
      -m mets \
      --database gtdb \
      --protein_extension .faa \
      --reference_dir gtdb \
      -o rnaspades.prokka \
      --CPUs 12 \
      -s \
      contigs || rc=$?
  
  gzip rnaspades.prokka/mets_full/diamond/*.out
  gzip rnaspades.prokka/taxonomy_counts/*.csv
  gzip rnaspades.prokka/taxonomy_estimation/*.out
  
  cat <<-END_VERSIONS > versions.yml
  "NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH":
      eukulele: $(echo $(EUKulele --version 2>&1) | sed -n 's/.* \([0-9]\+\.[0-9]\+\.[0-9]\+\).*/\1/p')
  END_VERSIONS
  
  if [ $rc -le 1 ]; then
      exit 0
  else
      exit $rc;
  fi

Command exit status:
  1

Command output:
  All reference files for GTDB downloaded to gtdb/gtdb
  Running EUKulele with command line arguments, as no valid configuration file was provided.
  Setting things up...
  Could not successfully install all external dependent software.
  Check DIAMOND, BLAST, BUSCO, and TransDecoder installation.
  ['proteins']
  Specified reference directory, reference FASTA, and protein map/taxonomy table not found. Using database in location: gtdb/gtdb.
  Automatically downloading database gtdb . If you intended to use an existing database folder, be sure a reference FASTA, protein map, and taxonomy table are provided. Check the documentation for details.

Command error:
    6300K .......... .......... .......... .......... .......... 77%  576M 0s
    6350K .......... .......... .......... .......... .......... 78% 1.24M 0s
    6400K .......... .......... .......... .......... .......... 79% 25.6M 0s
    6450K .......... .......... .......... .......... .......... 79% 42.0M 0s
    6500K .......... .......... .......... .......... .......... 80% 35.4M 0s
    6550K .......... .......... .......... .......... .......... 80% 73.3M 0s
    6600K .......... .......... .......... .......... .......... 81% 69.1M 0s
    6650K .......... .......... .......... .......... .......... 82% 35.2M 0s
    6700K .......... .......... .......... .......... .......... 82% 77.9M 0s
    6750K .......... .......... .......... .......... .......... 83% 35.5M 0s
    6800K .......... .......... .......... .......... .......... 84% 76.8M 0s
    6850K .......... .......... .......... .......... .......... 84% 55.5M 0s
    6900K .......... .......... .......... .......... .......... 85% 34.1M 0s
    6950K .......... .......... .......... .......... .......... 85% 76.5M 0s
    7000K .......... .......... .......... .......... .......... 86% 83.2M 0s
    7050K .......... .......... .......... .......... .......... 87% 35.0M 0s
    7100K .......... .......... .......... .......... .......... 87% 77.0M 0s
    7150K .......... .......... .......... .......... .......... 88% 30.6M 0s
    7200K .......... .......... .......... .......... .......... 88% 91.6M 0s
    7250K .......... .......... .......... .......... .......... 89% 34.4M 0s
    7300K .......... .......... .......... .......... .......... 90% 78.5M 0s
    7350K .......... .......... .......... .......... .......... 90% 75.7M 0s
    7400K .......... .......... .......... .......... .......... 91% 47.0M 0s
    7450K .......... .......... .......... .......... .......... 92% 77.3M 0s
    7500K .......... .......... .......... .......... .......... 92% 77.3M 0s
    7550K .......... .......... .......... .......... .......... 93% 30.0M 0s
    7600K .......... .......... .......... .......... .......... 93% 79.3M 0s
    7650K .......... .......... .......... .......... .......... 94% 77.5M 0s
    7700K .......... .......... .......... .......... .......... 95% 32.8M 0s
    7750K .......... .......... .......... .......... .......... 95% 74.1M 0s
    7800K .......... .......... .......... .......... .......... 96% 65.9M 0s
    7850K .......... .......... .......... .......... .......... 96% 77.3M 0s
    7900K .......... .......... .......... .......... .......... 97% 53.4M 0s
    7950K .......... .......... .......... .......... .......... 98% 55.7M 0s
    8000K .......... .......... .......... .......... .......... 98% 75.0M 0s
    8050K .......... .......... .......... .......... .......... 99% 66.6M 0s
    8100K .......... .......... .......... .......... .......... 99% 34.5M 0s
    8150K                                                       100% 1.76T=0.3s
  
  2024-06-13 15:05:29 (23.7 MB/s) - 'gtdb/gtdb/taxonomy-table.txt' saved [8346567/8346567]
  
  All reference files for GTDB downloaded to gtdb/gtdb
  Running EUKulele with command line arguments, as no valid configuration file was provided.
  Setting things up...
  Could not successfully install all external dependent software.
  Check DIAMOND, BLAST, BUSCO, and TransDecoder installation.
  ['proteins']
  Specified reference directory, reference FASTA, and protein map/taxonomy table not found. Using database in location: gtdb/gtdb.
  Automatically downloading database gtdb . If you intended to use an existing database folder, be sure a reference FASTA, protein map, and taxonomy table are provided. Check the documentation for details.
  gzip: rnaspades.prokka/mets_full/diamond/*.out: No such file or directory

Work dir:
  /home/erick/Nextflow/nf-core_metatdenovo/work/db/e28b136334298c1eb96640a9266473

Tip: view the complete command output by changing to the process work dir and entering the command `cat .command.out`

 -- Check '.nextflow.log' file for details
ERROR ~ Failed to invoke `workflow.onComplete` event handler

 -- Check script '/root/.nextflow/assets/nf-core/metatdenovo/./workflows/metatdenovo.nf' at line: 564 or see '.nextflow.log' file for more details
I understand that I have not downloaded all the files I need. I think I can solve this step I will let you know