Erick Delgadillo-Nuño
06/12/2024, 7:46 AMERROR ~ Error executing process > 'NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH (rnaspades.prokka)'
Caused by:
Process `NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH (rnaspades.prokka)` terminated with an error exit status (1)
Command executed:
rc=0
mkdir contigs
gunzip -c prokka.faa.gz > ./contigs/proteins.faa
EUKulele \
-m mets \
--database gtdb \
--protein_extension .faa \
--reference_dir eukulele \
-o rnaspades.prokka \
--CPUs 12 \
-s \
contigs || rc=$?
gzip rnaspades.prokka/mets_full/diamond/*.out
gzip rnaspades.prokka/taxonomy_counts/*.csv
gzip rnaspades.prokka/taxonomy_estimation/*.out
cat <<-END_VERSIONS > versions.yml
"NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH":
eukulele: $(echo $(EUKulele --version 2>&1) | sed -n 's/.* \([0-9]\+\.[0-9]\+\.[0-9]\+\).*/\1/p')
END_VERSIONS
if [ $rc -le 1 ]; then
exit 0
else
exit $rc;
fi
Command exit status:
1
Command output:
All reference files for GTDB downloaded to eukulele/gtdb
Running EUKulele with command line arguments, as no valid configuration file was provided.
Setting things up...
Could not successfully install all external dependent software.
Check DIAMOND, BLAST, BUSCO, and TransDecoder installation.
['proteins']
Specified reference directory, reference FASTA, and protein map/taxonomy table not found. Using database in location: eukulele/gtdb.
Automatically downloading database gtdb . If you intended to use an existing database folder, be sure a reference FASTA, protein map, and taxonomy table are provided. Check the documentation for details.
Command error:
mkdir: can't create directory 'eukulele/': No such file or directory
mkdir: can't create directory 'eukulele/': No such file or directory
eukulele/gtdb/reference.pep.fa: No such file or directory
eukulele/gtdb/taxonomy-table.txt: No such file or directory
All reference files for GTDB downloaded to eukulele/gtdb
Running EUKulele with command line arguments, as no valid configuration file was provided.
Setting things up...
Could not successfully install all external dependent software.
Check DIAMOND, BLAST, BUSCO, and TransDecoder installation.
['proteins']
Specified reference directory, reference FASTA, and protein map/taxonomy table not found. Using database in location: eukulele/gtdb.
Automatically downloading database gtdb . If you intended to use an existing database folder, be sure a reference FASTA, protein map, and taxonomy table are provided. Check the documentation for details.
gzip: rnaspades.prokka/mets_full/diamond/*.out: No such file or directory
Work dir:
/home/erick/Nextflow/nf-core_metatdenovo/work/b5/d67099bed08afe34efc734777ae227
Tip: you can try to figure out what's wrong by changing to the process work dir and showing the script file named `.command.sh`
-- Check '.nextflow.log' file for detailsDanilo Di Leo
06/12/2024, 2:17 PMErick Delgadillo-Nuño
06/13/2024, 9:13 AMErick Delgadillo-Nuño
06/13/2024, 9:13 AMErick Delgadillo-Nuño
06/13/2024, 9:13 AMErick Delgadillo-Nuño
06/13/2024, 9:14 AMErick Delgadillo-Nuño
06/13/2024, 9:14 AMDanilo Di Leo
06/13/2024, 9:53 AMErick Delgadillo-Nuño
06/13/2024, 3:15 PM--eukulele_dbpath /home/erick/Descargas/gtdb/Erick Delgadillo-Nuño
06/13/2024, 3:15 PMErick Delgadillo-Nuño
06/13/2024, 3:16 PMERROR ~ Error executing process > 'NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH (rnaspades.prokka)'
Caused by:
Process `NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH (rnaspades.prokka)` terminated with an error exit status (1)
Command executed:
rc=0
mkdir contigs
gunzip -c prokka.faa.gz > ./contigs/proteins.faa
EUKulele \
-m mets \
--database gtdb \
--protein_extension .faa \
--reference_dir gtdb \
-o rnaspades.prokka \
--CPUs 12 \
-s \
contigs || rc=$?
gzip rnaspades.prokka/mets_full/diamond/*.out
gzip rnaspades.prokka/taxonomy_counts/*.csv
gzip rnaspades.prokka/taxonomy_estimation/*.out
cat <<-END_VERSIONS > versions.yml
"NFCORE_METATDENOVO:METATDENOVO:SUB_EUKULELE:EUKULELE_SEARCH":
eukulele: $(echo $(EUKulele --version 2>&1) | sed -n 's/.* \([0-9]\+\.[0-9]\+\.[0-9]\+\).*/\1/p')
END_VERSIONS
if [ $rc -le 1 ]; then
exit 0
else
exit $rc;
fi
Command exit status:
1
Command output:
All reference files for GTDB downloaded to gtdb/gtdb
Running EUKulele with command line arguments, as no valid configuration file was provided.
Setting things up...
Could not successfully install all external dependent software.
Check DIAMOND, BLAST, BUSCO, and TransDecoder installation.
['proteins']
Specified reference directory, reference FASTA, and protein map/taxonomy table not found. Using database in location: gtdb/gtdb.
Automatically downloading database gtdb . If you intended to use an existing database folder, be sure a reference FASTA, protein map, and taxonomy table are provided. Check the documentation for details.
Command error:
6300K .......... .......... .......... .......... .......... 77% 576M 0s
6350K .......... .......... .......... .......... .......... 78% 1.24M 0s
6400K .......... .......... .......... .......... .......... 79% 25.6M 0s
6450K .......... .......... .......... .......... .......... 79% 42.0M 0s
6500K .......... .......... .......... .......... .......... 80% 35.4M 0s
6550K .......... .......... .......... .......... .......... 80% 73.3M 0s
6600K .......... .......... .......... .......... .......... 81% 69.1M 0s
6650K .......... .......... .......... .......... .......... 82% 35.2M 0s
6700K .......... .......... .......... .......... .......... 82% 77.9M 0s
6750K .......... .......... .......... .......... .......... 83% 35.5M 0s
6800K .......... .......... .......... .......... .......... 84% 76.8M 0s
6850K .......... .......... .......... .......... .......... 84% 55.5M 0s
6900K .......... .......... .......... .......... .......... 85% 34.1M 0s
6950K .......... .......... .......... .......... .......... 85% 76.5M 0s
7000K .......... .......... .......... .......... .......... 86% 83.2M 0s
7050K .......... .......... .......... .......... .......... 87% 35.0M 0s
7100K .......... .......... .......... .......... .......... 87% 77.0M 0s
7150K .......... .......... .......... .......... .......... 88% 30.6M 0s
7200K .......... .......... .......... .......... .......... 88% 91.6M 0s
7250K .......... .......... .......... .......... .......... 89% 34.4M 0s
7300K .......... .......... .......... .......... .......... 90% 78.5M 0s
7350K .......... .......... .......... .......... .......... 90% 75.7M 0s
7400K .......... .......... .......... .......... .......... 91% 47.0M 0s
7450K .......... .......... .......... .......... .......... 92% 77.3M 0s
7500K .......... .......... .......... .......... .......... 92% 77.3M 0s
7550K .......... .......... .......... .......... .......... 93% 30.0M 0s
7600K .......... .......... .......... .......... .......... 93% 79.3M 0s
7650K .......... .......... .......... .......... .......... 94% 77.5M 0s
7700K .......... .......... .......... .......... .......... 95% 32.8M 0s
7750K .......... .......... .......... .......... .......... 95% 74.1M 0s
7800K .......... .......... .......... .......... .......... 96% 65.9M 0s
7850K .......... .......... .......... .......... .......... 96% 77.3M 0s
7900K .......... .......... .......... .......... .......... 97% 53.4M 0s
7950K .......... .......... .......... .......... .......... 98% 55.7M 0s
8000K .......... .......... .......... .......... .......... 98% 75.0M 0s
8050K .......... .......... .......... .......... .......... 99% 66.6M 0s
8100K .......... .......... .......... .......... .......... 99% 34.5M 0s
8150K 100% 1.76T=0.3s
2024-06-13 15:05:29 (23.7 MB/s) - 'gtdb/gtdb/taxonomy-table.txt' saved [8346567/8346567]
All reference files for GTDB downloaded to gtdb/gtdb
Running EUKulele with command line arguments, as no valid configuration file was provided.
Setting things up...
Could not successfully install all external dependent software.
Check DIAMOND, BLAST, BUSCO, and TransDecoder installation.
['proteins']
Specified reference directory, reference FASTA, and protein map/taxonomy table not found. Using database in location: gtdb/gtdb.
Automatically downloading database gtdb . If you intended to use an existing database folder, be sure a reference FASTA, protein map, and taxonomy table are provided. Check the documentation for details.
gzip: rnaspades.prokka/mets_full/diamond/*.out: No such file or directory
Work dir:
/home/erick/Nextflow/nf-core_metatdenovo/work/db/e28b136334298c1eb96640a9266473
Tip: view the complete command output by changing to the process work dir and entering the command `cat .command.out`
-- Check '.nextflow.log' file for details
ERROR ~ Failed to invoke `workflow.onComplete` event handler
-- Check script '/root/.nextflow/assets/nf-core/metatdenovo/./workflows/metatdenovo.nf' at line: 564 or see '.nextflow.log' file for more detailsErick Delgadillo-Nuño
06/13/2024, 3:17 PM