• Hello everyone, I have a question, maybe it is a bit stupid, but I would like to know if STAR is in the two-pass annotation guided configuration or if it can be added. So far, I have not found any information about it. I would be very grateful if you could guide me please.

STAR runs with `--twopassMode Basic`: https://github.com/nf-core/rnasplice/blob/1d0494ae3402d1a46e0adadad24f81a0ff855c77/conf/modules.config#L246

Ohh, I'm sorry, I hadn't found it. Thank you!