What type of results are obtained in `dexseq_exon/...
# rnasplice
s
What type of results are obtained in
dexseq_exon/counts/*.clean.count.txt
, are they normalized data? Or raw counts
t
Those are the output of
dexseq_count.py
, I think it just counts the reads overlapping exon parts, without any scaling/normalization.
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a
I believe I did not get any results
Apology, when I run the command, I get only 4 folders as an output 1-contrastsheet, 2- Fastqc, 3- pipelineinfo 4- trimglore thats it, non of the results contains dexseq_counts
I am new to this workflow and please my apology in advance, but please be patience with me
t
What's you command line? You'll need to specify tools, e.g.
--dexseq_exon
.
a
nextflow run nf-core/rnasplice --input /home/ahmed/data/mRNA_E_cig/samplesheet.csv --contrasts /home/ahmed/data/mRNA_E_cig/contrastsheet.csv --outdir /home/ahmed/nextflow --genome GRCm38 --skip_alignment --pseudo_aligner salmon -profile docker --max_memory 20.GB
This was my command line
I did not specify
That might be the reason for the error!
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