Felix Krueger
03/22/2024, 11:14 AMError executing process > 'NFCORE_FETCHNGS:SRA:FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS:SRATOOLS_FASTERQDUMP (SRX3445732_SRR6348978)'
Caused by:
Essential container in task exited
Command executed:
export NCBI_SETTINGS="$PWD/user-settings.mkfg"
fasterq-dump \
--split-files --include-technical \
--threads 6 \
--outfile SRX3445732_SRR6348978 \
\
SRR6348978
pigz \
\
--no-name \
--processes 6 \
*.fastq
cat <<-END_VERSIONS > versions.yml
"NFCORE_FETCHNGS:SRA:FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS:SRATOOLS_FASTERQDUMP":
sratools: $(fasterq-dump --version 2>&1 | grep -Eo '[0-9.]+')
pigz: $( pigz --version 2>&1 | sed 's/pigz //g' )
END_VERSIONS
Command exit status:
1
Command output:
(empty)
Command error:
spots read : 339,247,891
reads read : 339,247,891
reads written : 339,247,891
pigz: skipping: *.fastq does not exist
3:24PM INF shutdown filesystem start
3:25PM INF shutdown filesystem done
So it appears that fasterq-dump has successfully extracted reads, but pigz then dies as there are no files called .fastq , is that correct? I went to the work directory where of the failed process, here are the contents:
fasterq.tmp.ip/
2024-03-20 15:05:58 0
2024-03-20 15:06:13 0 .command.begin
2024-03-20 15:24:32 129 .command.err
2024-03-20 15:24:32 129 .command.log
2024-03-20 15:25:38 0 .command.out
2024-03-20 15:05:58 11027 .command.run
2024-03-20 15:05:58 528 .command.sh
2024-03-20 15:06:13 0 .command.trace
2024-03-20 15:24:32 1 .exitcode
2024-03-20 15:25:25 4263995 .fusion.log
2024-03-20 15:24:32 29 .fusion.symlinks
2024-03-20 15:24:32 96 SRR6348978
2024-03-20 15:24:34 95223460854 SRX3445732_SRR6348978
2024-03-20 15:24:32 104 user-settings.mkfg
That SRX… files is indeed in FastQ format:
head SRX3445732_SRR6348978
@SRR6348978.1 1 length=98
TTGTACTAAGGTCAAATCTAAGTGGATTAAGGAACTCCACATAAAACCAGAGACACTGAAACTTACAGAGGAGAAAGTAGGGAAAAGCCTCGAAGATA
+SRR6348978.1 1 length=98
A<7F<JFJJJFJJJJJJJJJJJAFAJFJJJJJJJFJJJJJJJJJJJJJJFFFJFJFFFJJJJFJ<JFJFFJFJJJFJFFJFJJJJFJJ<JFFFJJJJF
@SRR6348978.2 2 length=98
CCAGGTCCATCCATTTGGCTAGGAATTTCATAAATTCATTCTTTTTAATAGCTGAGTAGTACTCCATTGTGTAGATCTACCACATTTTCTGTATCCAT
+SRR6348978.2 2 length=98
A<FFFJJJJJJJJFJJJJJJJJJFJFJJJJJJJJJJJJJJJFJJJJJJJJJJJJJJJJJJJJFFJJJJJJJFJJJJ-A----77A<--<7-7------
Any clues on how to make this work?