Hello everyone. I'm running into an issue with th...
# rnasplice
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Hello everyone. I'm running into an issue with the pipeline where the rmats workflow is getting skipped and I can't figure out why. I read the docs and I believe I have the appropriate parameters set so that the workflow will run. Below is my params file that I specify when launching the pipeline. Anyone have any suggestions why rmats wouldn't run? I see nothing in the .nextflow.log file about rmats.
{
"input": "mouse_sample_sheet1.csv",
"outdir": "<s3://XXXX/rnaseq/mouse_rnasplice/cortex/20240305_1>",
"gtf": "<s3://XXXX/ref/tg_mouse_hybrid_gtf_v2_2024-01-16.gtf>",
"fasta": "<s3://XXXX/ref/tg_mouse_hybrid_reference_v2.fa>",
"save_reference": true,
"contrasts": "contrasts1.csv",
"sashimi_plot": false,
"rmats": true,
"aligner": "star_salmon",
"dexseq_dtu": true,
"suppa": true,
"rmats_read_len": 151,
"rmats_paired_stats": false
}