Yucheng Zhang
03/01/2024, 2:32 PM1.12.0 in the fasterq-dump step. I am wondering whether anyone has similar issues. Here is the command I used nextflow run nf-core/fetchngs -r 1.12.0 --input samplesheet.csv --outdir fetchngsout -profile tufts --download_method sratools . My samplesheet.csv only has one identifier GSE80182 . Below is the error message
ERROR ~ Error executing process > 'NFCORE_FETCHNGS:SRA:FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS:SRATOOLS_FASTERQDUMP (SRX1693957_SRR3362667)'
Caused by:
Process `NFCORE_FETCHNGS:SRA:FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS:SRATOOLS_FASTERQDUMP (SRX1693957_SRR3362667)` terminated with an error exit status (3)
Command executed:
export NCBI_SETTINGS="$PWD/user-settings.mkfg"
fasterq-dump \
--split-files --include-technical \
--threads 6 \
--outfile SRX1693957_SRR3362667 \
\
SRR3362667
pigz \
\
--no-name \
--processes 6 \
*.fastq
cat <<-END_VERSIONS > versions.yml
"NFCORE_FETCHNGS:SRA:FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS:SRATOOLS_FASTERQDUMP":
sratools: $(fasterq-dump --version 2>&1 | grep -Eo '[0-9.]+')
pigz: $( pigz --version 2>&1 | sed 's/pigz //g' )
END_VERSIONS
Command exit status:
3
Command output:
(empty)
Command error:
2024-03-01T14:11:02 fasterq-dump.2.11.0 err: invalid accession 'SRR3362667'
fasterq-dump quit with error code 3
Work dir:
/cluster/home/tutln02/nf-core_workshp/1.12.0/work/db/35ade848d7ce0b53d4db4b492c8bc3
Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
-- Check '.nextflow.log' file for details